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CIMA™

CIMA is a hyperspectral confocal system sensitive between 400 nm and 1700 nm.

Description

Confocal Hyperspectral Microscope

CIMA is a hyperspectral confocal system sensitive between 400 and 1700 nm. Spectral resolution can be as low as 0.2 nm in the visible range, and 0.6 nm in the infrared. It pairs a galvanometer head and one of the fastest and most sensitive cameras on the market to yield an acquisition rate above 300 spectra per second. CIMA provides three acquisition modes: confocal hyperspectral imaging, multispectral fluorescence imaging, and emission spectroscopy of a sample in a cuvette.

CIMA Applications Overview

  • Simultaneously study the spatial distribution and spectral properties of complex nanomaterials such as upconverting nanoparticles and quantum dots.
  • Perform in vitro or in vivo high resolution hyperspectral microscopy.
  • Develop new sensors based on environment dependent spectral shifting of their fluorescence signal.

Technology

  • Hyperspectral microscopy

Fields

  • Life Science
  • Material Science

Techincal Specifications

Technical Specifications

VIS IR
Spectral range 400-1700 nm 400-1700 nm
Spectral resolution (FWHM) < 0.2 nm (Custom resolution available upon request) < 0.6 nm (Custom resolution available upon request)
Spatial resolution Diffraction Limited Diffraction Limited
Cameras Back-illuminated CCD or EMCCD InGaAs linear array
Excitation wavelength 980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W. 980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W.
Microscope Scientific grade, inverted Scientific grade, inverted
Confocal Mode
VIS IR
Scanning region 300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective) 300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective)
Pinhole diameter 30 to 200 µm 30 to 200 µm
Cuvette Mode
VIS IR
Cuvette size Standard 10 mm x 10 mm (3.5 mL) Standard 10 mm x 10 mm (3.5 mL)
Fluorescence Mode
VIS IR
Camera Megapixel colour camera for fluorescence and sample visualization Megapixel colour camera for fluorescence and sample visualization
Illumination lamp UHP mercury lamp 130W UHP mercury lamp 130W
Epifluorescence filter cubes Up to 6 different filter cubes Up to 6 different filter cubes

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