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CIMA™

CIMA is a hyperspectral confocal system sensitive between 400 nm and 1700 nm.

Description

Confocal Hyperspectral Microscope

CIMA is a hyperspectral confocal system sensitive between 400 and 1700 nm. Spectral resolution can be as low as 0.2 nm in the visible range, and 0.6 nm in the infrared. It pairs a galvanometer head and one of the fastest and most sensitive cameras on the market to yield an acquisition rate above 300 spectra per second. CIMA provides three acquisition modes: confocal hyperspectral imaging, multispectral fluorescence imaging, and emission spectroscopy of a sample in a cuvette.

CIMA Applications Overview

  • Simultaneously study the spatial distribution and spectral properties of complex nanomaterials such as upconverting nanoparticles and quantum dots.
  • Perform in vitro or in vivo high resolution hyperspectral microscopy.
  • Develop new sensors based on environment dependent spectral shifting of their fluorescence signal.

Technology

  • Hyperspectral microscopy

Fields

  • Life Science
  • Material Science

Techincal Specifications

Technical Specifications

VISIR
Spectral range400-1700 nm400-1700 nm
Spectral resolution (FWHM)< 0.2 nm (Custom resolution available upon request)< 0.6 nm (Custom resolution available upon request)
Spatial resolutionDiffraction LimitedDiffraction Limited
CamerasBack-illuminated CCD or EMCCDInGaAs linear array
Excitation wavelength980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W.980 nm (other wavelengths available). 3 laser input ports. UHP mercury lamp 130W.
MicroscopeScientific grade, invertedScientific grade, inverted
Confocal Mode
VISIR
Scanning region300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective)300 µm x 300 µm (20X objective), 100 µm x 100 µm (60X objective)
Pinhole diameter30 to 200 µm30 to 200 µm
Cuvette Mode
VISIR
Cuvette sizeStandard 10 mm x 10 mm (3.5 mL)Standard 10 mm x 10 mm (3.5 mL)
Fluorescence Mode
VISIR
CameraMegapixel colour camera for fluorescence and sample visualizationMegapixel colour camera for fluorescence and sample visualization
Illumination lampUHP mercury lamp 130WUHP mercury lamp 130W
Epifluorescence filter cubesUp to 6 different filter cubesUp to 6 different filter cubes

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